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At least four times the forexexchange co uk space should be used for sizing, to Index 379 Horseradish peroxidase, 150-151 HPA, see Hybridization protection assay HPLC, 228, 343,344 HPV, see Human papillomavirus Human immunodeficiency virus assessment, 214-215 CD4 counts, 204 clinical aspects, 141-142, 183, 185 detection bDNA signal amplification, 132-134, 141-142, 204 ligase chain reaction, 318 NASBA, 270-271 PCR, 19, 183-189 PCR in situ hybridization, 238, 239-240, 251,253 RT-PCR, 66-71, 194-195, 198, 203-205, 205,211-212, 214-215 RT-PCR in situ hybridization, 251,253 self-sustained sequence replication, 293-294, 295-296, 298-299, 301-311 sensitivity, 152 threshold, 153 drug resistant, 298-299 histology, 253 infection status, 251,253 pathogenesis, 238 prognostic indicator, 213-215,214-215 quantification, 213-214 sample preparation, 142-143 Human papillomavirus detection methods, 92 in situ hybridization, 85-91 ligation-activated transcription, 362, 365-367, 369-371 multiplex PCR, 221 PCR in situ hybridization, 249-250 E7, 369 histology, 253 lesions, 250 multiple infection, 92 viral typing, 165-166, 318-310 Human T-lymphotropic virus type I detection by ligase chain reaction, 318 viral typing, 319 Human T-lymphotropic virus type II, 319 Hybridization, see also specific detection methods antibody-capture solution, 353-354 in bDNA signal amplification, 135-137 bead-based sandwich, 295-296, 298-299, 307-309 application, 312 in chemiluminescence detection methods, 151,155, 166 cross-hybridization, 92 deoxyribonucleic acid, forexexchange co uk filter, 56, 62-64, 239 kinetics, 56-57 melting temperature, 56-57 M-PCR, 228 in multiplex PCR, 233 Northern blot, 62-64 nucleic acid, 351 protocol, 160, 164-165 for self-sustained sequence replication, 295-296 sensitivity, 63 solid-phase, 228 solution method, 163 Southern blot, 227-228 stringency, 63, 64, 310 theory, 55-57 Hybridization, bead-based sandwich, 295-296 Hybridization, in situ, 75-97, 239, 243,251, 253 advantages, 76, 97 applications, 85-91, 94-95, 97 chemiluminescence detection methods, 163-166 controls, 84-85, 91 denaturation, 93 description, 76 diffusion, 243 digestion, 92-93, 93 equipment, 85, 88 immunohistochemistry, 94 kinetics, 80-81 limitations, 94 nonspecific signal, 84, 91, 93-94 PCR, 96 prehybridization, 79-80 probe labeling, 81-83 nonisotopic, 76-77 product detection, 83-84, 90-91 protocol, 86-91, 90, 243 results, 92 RT-PCR, 96 Mader: Understanding I.
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